PCR and preparing for eDNA sequencing

May 24, 2016

Hello citizen scientists! Here's another update from the lab.

 

We've finally extracted eDNA from samples collected from nearly a full year of sampling at Pigpen Branch! Now, we're performing 'PCR' (polymerase chain reaction), which replicates that DNA inside a test tube. Pictured here are a little more than half the samples getting ready for PCRs. Woo! 

 

After these PCRs, we'll pool our samples together for sequencing on a 'next generation sequencing' platform. From these sequence data, we can bioinformatically infer what species are present in the stream. Stay tuned for results! 

 

 

 

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