First eDNA results from Tremont

August 20, 2015

Hey folks! We've just received sequences from our first sampling at the Tremont streams.

 

Unfortunately, we encountered a few complications while amplifying the DNA (we're working on fixing these for the next batch of samples!), but here's a little teaser from a Pigpen Branch sample collected in June. The chart shows the proportion of sequences that matched each salamander species. It uses scientific names, but here are the common names associated with each species:

 

Desmognathus quadramaculatus: black-bellied salamander

Eurycea wilderae: Blue Ridge two-lined salamander

Gyrinophilus porphyriticus: spring salamander

Desmognathus monticola: seal salamander

 

Note that some of the DNA didn't match well to any of the sequences we already had in our reference library. We can tell that they belong to the genera Eurycea and Desmognathus, but aren't quite sure which species. We'll have to figure out that mystery later!

 

We've now completed sampling in July and August, and we're hoping to sequence those samples soon. Stay tuned for more updates!

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